Fig. 10
The effect of C60 fullerenes and their nanocomplexes with anticancer drugs Cis and Dox on intracellular ROS generation in U937 cells. U937 cells were treated with mentioned preparations for 30 min, then were stained with carboxy-H2DCFDA (see “Methods”). The fluorescence intensities of 10,000 cells were then analyzed by BD-FACS Calibur. All results are expressed as mean ± SD of three independent experiments. *p < 0.05 compared to basal level of ROS generation in control; #p < 0.05 compared to corresponding cells without treatment with PMA, as analyzed by unpaired t test