Table 1 Stepwise optimization of variable parameters
From: Nanolipid carrier-based thermoreversible gel for localized delivery of docetaxel to breast cancer
| Step | Formulation code | Variable parameters | Response parameters | Opt. parameter | Level of opt (code) | ||||
|---|---|---|---|---|---|---|---|---|---|
| Solid lipid (mg) | PluronicF68 (mg) | Homogenization time (min) | Sonication time (min) | Size range (nm) (Opta) | PDI range (Optb) | ||||
| Step 1 | F1–F5 | 200 | 200 | 1–5 | – | 2,023–1,384 (1,023 ± 58.32) | 0.321–0.202 (0.202 ± 0.44) | Homogenization time | 3 min (F3) |
| Step 2 | F6–F10 | 200 | 100–250 | 3 | 3 | 291.8–158.6 (101.3 ± 49.21) | 0.363–0.202 (0.202 ± 0.004) | Surfactant concentration (F68) | 200 mg (F9) |
| Step 3 | F10–F15 | 200 | 200 | 3 | 2–5 | 368.4–168.1 (136.1 ± 4.16) | 0.365–0.217 (0.217 ± 0.003) | Sonication time | 4 min (F14) |